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Histone H4 acetyl-methyllysine marks accessible chromatin that resists compaction

GSE326873 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 95 samples Submitted 2026/04/22 Platform GPL34284
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 95 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Homo sapiens (human)
Samples
95 samples: 38 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Histone H4 acetyl-methyllysine marks accessible chromatin that resists compactionbioRxiv : the preprint server for biology · 2026
Summary (written by the submitters)
Regulatory DNA often remains accessible even under conditions of widespread chromatin compaction. Specific factors and histone modifications mark these regions and help maintain local accessibility. Here, we examine the genomic landscape of acetyl-methyllysine (Kacme), a recently discovered histone post-translational modification. Across multiple systems, Kacme is highly enriched at sites of accessible chromatin, including active promoters, enhancers, silencers, and CTCF-binding sites.
Read the full summary ↓
We find that Kacme is selectively retained at loci that resist condensation during mitosis, marks XIST and escapee regions on the inactive X chromosome in female cells, and demarcates the boundaries of broad heterochromatin domains. Using the KDM6A/6B inhibitor GSK-J4 to elevate H3K27me3 levels, we show that Kacme-marked insulator elements block heterochromatin spreading and protect adjacent genes from transcriptional repression. Taken together, our findings define the chromatin features associated with Kacme and support a model in which Kacme safeguards chromatin accessibility, particularly at loci that remain active despite compaction and silencing pressures.
Published in
Histone H4 acetyl-methyllysine marks accessible chromatin that resists compaction
Pintado-Urbanc AP, Brown CL, Connor LJ et al. · bioRxiv : the preprint server for biology 2026 · PMID 42079196 · doi:10.64898/2026.04.17.718779
This dataset
Open GSE326873 in finder → Find more human ChIP / ATAC / CUT&Tag datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE326873_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1447509 and SRA study SRP688945. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42079196) ↗
Study design
38 conditions, mostly in duplicate
TimeLapse-seq_Mitotic_T0_HEK293T_ys4U ×3 TimeLapse-seq_Mitotic_T40_HEK293T_ys4U ×3 TimeLapse-seq_Mitotic_T105_HEK293T_ys4U ×3 TimeLapse-seq_Mitotic_T300_HEK293T_ys4U ×3 TimeLapse-seq_Async_HEK293T_ys4U ×3 CUT&RUN_HEK293T_CTCF ×2 ATAC_Mitotic_T0_HEK293T ×2 ATAC_Mitotic_T40_HEK293T ×2 +44 more

Supports a between-group comparison across 81 samples.

How we read this: 38 replicated groups read from 95 sample titles; they account for 81 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 55 more — browse all 95 samples with per-sample file links →

Datasets on a similar topic

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