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Early macrophage immune responses and pathogenic features of an emerging Mycobacterium wolinskyi

GSE327535 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/06/05 Platform GPL24247
Summary
Mycobacterium wolinskyi is an emerging rapidly growing mycobacterial species whose pathogenic mechanisms and host response patterns remain poorly understood. In this study, a clinical M. wolinskyi strain, designated QL01, was isolated from an 11-year-old child with a facial skin infection. Whole-genome sequencing revealed multiple features associated with pathogenicity, antimicrobial resistance, and host adaptation, including antimicrobial resistance genes, putative virulence factors, genomic islands, prophages, type VII secretion systems, and mammalian cell entry operons. To investigate the early host response, mouse bone marrow-derived macrophages were infected with M. wolinskyi QL01 and subjected to RNA sequencing. Transcriptomic analysis showed broad induction of inflammatory and immunometabolic programs, with prominent enrichment of the TNF, NF-kappa B, and HIF-1 signaling pathways. These findings provide a genomic and transcriptomic framework for understanding host-pathogen interactions involving M. wolinskyi.
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Direct links to NCBI, no account and no request form: the whole study as GSE327535_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1451178 and SRA study SRP690602. Searching any of these in the dataset finder brings you back here.

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