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RNA-seq profiling of iPSC-derived microglia (iMicroglia) with wild-type or GPNMB knockout genotype

GSE328042 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/05/06 Platform GPL30173
Summary
Glycoprotein nonmetastatic melanoma B (GPNMB) is critical to cellular uptake of pathological forms of alpha-synuclein (aSyn), the hallmark disease protein in Parkinson's Disease (PD). Here, we demonstrate that the non-membrane-anchored, extracellular domain of GPNMB can function in a non-cell-autonomous manner. In human brain, GPNMB is widely expressed in neurons and microglia. In induced pluripotent stem cell-derived microglia (iMicroglia), GPNMB expression and secretion increase with exposure to apoptotic neurons. In the aSyn fibril-seeded model of PD, iMicroglia-derived GPNMB enhances neuronal aSyn uptake and development of aSyn pathology, including in GPNMB knockout neurons. This dataset contains bulk RNA-seq data from iPSC-derived microglia (iMicroglia) differentiated using the Brownjohn protocol from isogenic GPNMB wild-type (WT) and GPNMB knockout (KO) iPSC lines (3 biological replicates each), treated with PBS as vehicle control.
Published in
Secreted GPNMB enhances uptake of fibrillar alpha-synuclein in a non-cell-autonomous process that can be blocked by anti-GPNMB antibodies
Carceles-Cordon M, Brody EM, Boucher ML et al. · Neuron 2026 · PMID 42127911 · doi:10.1016/j.neuron.2026.04.033
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Also filed as BioProject PRJNA1453554 and SRA study SRP691849. Searching any of these in the dataset finder brings you back here.

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