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Resolving METTL5 Specificity: Direct RNA Sequencing Reveals No Evidence for METTL5 mediated mRNA m6A Methylation in mESCs

GSE328291 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2026/05/19 Platform GPL24973
Summary
The methyltransferase-like 5 (METTL5) has been shown to catalyze m6A deposition on 18S ribosomal RNA. However, whether it also methylates mRNAs remains unclear. To address this, we employed direct RNA sequencing (ONT) for m6A detection on native mRNA molecules. We first validated the quantitative detection of m6A by ONT by treating mESCs with a METTL3 inhibitor. We then compared methylation levels of m6A sites between Mettl5-KO and WT mESCs. Our analysis provides no compelling evidence for METTL5 mediated mRNA methylation in vivo, indicating that its activity is restricted to rRNA.
Published in
Resolving METTL5 Specificity: Direct RNA Sequencing Reveals No Compelling Evidence for METTL5 mediated mRNA m6A Methylation in mESCs
Le Cam S, Valenta M, Schneider R · microPublication biology 2026 · PMID 42181001 · doi:10.17912/micropub.biology.002169
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Also filed as BioProject PRJNA1454497. Searching any of these in the dataset finder brings you back here.

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