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Histone Demethylase KDM6B Promotes Postnatal Oligodendrocyte Development and Cortical Myelination

GSE328562 Mus musculus (mouse) RNA-seq (gene expression) 24 samples Submitted 2026/05/04 Platform GPL24247Platform GPL34475
At a glance

RNA-seq (gene expression) · mouse · 24 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
24 samples: 6 × RNAseq_kdm6bWT_p1 vs 6 × RNAseq_kdm6bKO_p1 vs 6 × RNAseq_Kdm6bWT_p30 vs 6 × RNAseq_kdm6bKO_p30Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Histone demethylase KDM6B promotes postnatal oligodendrocyte maturation and cortical myelinationFrontiers in cellular neuroscience · 2026
Summary (written by the submitters)
Myelination in the postnatal cortex requires epigenetic programs that activate oligodendrocyte gene networks. Here we show that the histone H3K27me3 demethylase KDM6B promotes this process in vivo. Conditional Kdm6b deletion (Kdm6b-cKO) in Emx1⁺ dorsal telencephalic progenitors caused delayed cortical myelination. Lineage-specific RNA-seq revealed that the oligodendrocyte master regulator Sox10 was significantly reduced, as well as myelination effectors, indicating impaired oligodendrocyte maturation.
Read the full summary ↓
Chromatin immunoprecipitation assays of the same lineage showed increased H3K27me3 at promoter-proximal regions of Sox10, consistent with loss of KDM6B-mediated de-repression. Temporally controlled Sox10 induction partially rescued myelination in Kdm6b-cKO mice. These findings define a KDM6B→SOX10 axis that licenses oligodendrocyte development and cortical myelination by removing repressive H3K27me3 and sustaining active chromatin, with implications for KDM6B-related neurodevelopmental disorders.
Published in
Histone demethylase KDM6B promotes postnatal oligodendrocyte maturation and cortical myelination
Lambries R, Shen Z, Mias GI et al. · Frontiers in cellular neuroscience 2026 · PMID 42239646 · doi:10.3389/fncel.2026.1832157
This dataset
Open GSE328562 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE328562_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1455659 and SRA study SRP693366. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42239646) ↗
Study design
6 × RNAseq_kdm6bWT_p1 vs 6 × RNAseq_kdm6bKO_p1 vs 6 × RNAseq_Kdm6bWT_p30 vs 6 × RNAseq_kdm6bKO_p30

Supports a between-group comparison across 24 samples.

How we read this: 4 replicated groups read from 24 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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