Single-nucleus RNA-seq analysis of whole brains from 5xFAD mice with microglia-specific CD31 knockdown

We performed single-nucleus RNA sequencing (snRNA-seq) on whole-brain tissues from 5xFAD mice with microglia-specific CD31 knockdown to investigate transcriptional alterations associated with Alzheimer’s disease pathology. snRNA-seq analysis identified cell-type-specific transcriptional changes across multiple brain cell populations, including microglia, revealing that CD31 knockdown attenuated disease-associated microglial signatures and inflammatory responses while promoting pathways related to Aβ clearance. These findings provide insights into the role of microglial CD31 in regulating neuroinflammation and Alzheimer’s disease progression.