Establishment and validation of the NEX-RiboTag system for profiling the excitatory neuronal translatome in the postnatal mouse forebrain
Single-cell RNA-seq · mouse · 11 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Mus musculus (mouse)
- Samples
- 11 samples: 11 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Establishment and validation of the NEX-RiboTag system for profiling the excitatory neuronal translatome in the postnatal mouse forebrainNeuroscience · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE331310_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1467668 and SRA study SRP701688. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 11 sample titles: 11 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM9743685 Whole-tissue lysate RNA from mice cortex and hippocampus at P7, replicate 1 [1w-Cre-Input]
- GSM9743686 Whole-tissue lysate RNA from mice cortex and hippocampus at P7, replicate 2 [1w-WT-Input]
- GSM9743687 TRAP-enriched excitatory neuron RNA from mice cortex and hippocampus at P7, replicate 1 [1w-Cre-1]
- GSM9743688 TRAP-enriched excitatory neuron RNA from mice cortex and hippocampus at P7, replicate 2 [1w-Cre-2]
- GSM9743689 TRAP-enriched excitatory neuron RNA from mice cortex and hippocampus at P7, replicate 3 [1w-Cre-3]
- GSM9743690 TRAP-enriched excitatory neuron RNA from mice cortex and hippocampus at P14, replicate 1 [2w-Cre-1]
- GSM9743691 TRAP-enriched excitatory neuron RNA from mice cortex and hippocampus at P14, replicate 2 [2w-Cre-2]
- GSM9743692 TRAP-enriched excitatory neuron RNA from mice cortex and hippocampus at P14, replicate 3 [2w-Cre-3]
- GSM9743693 TRAP-enriched excitatory neuron RNA from mice cortex and hippocampus at P21, replicate 1 [3w-Cre-1]
- GSM9743694 TRAP-enriched excitatory neuron RNA from mice cortex and hippocampus at P21, replicate 2 [3w-Cre-2]
- GSM9743695 TRAP-enriched excitatory neuron RNA from mice cortex and hippocampus at P21, replicate 3 [3w-Cre-3]
- GSE325852 Translatome profiling reveals opposing alterations in inhibitory and excitatory neurons of Fragile X mice and identifies EPAC2 as a therapeutic target 69 samples shares translatome, excitatory
- GSE272272 Changes in the astrocyte translatome induced by developmental ethanol mediate dysregulation of neuronal development: role of the extracellular matrix and relevance to Fetal Alcohol Spectrum Disorders 48 samples shares translatome, neuronal
- GSE253438 A transient brain endothelial translatome response to endotoxin is associated with mild cognitive changes post-shock in young mice 48 samples shares translatome
- GSE273088 Ribo-lite, a method defining paired transcriptome (RNA-seq) and translatome (Ribo-seq) at low input on AML cells from mice treated with 5+3 or vehicle and fed standard amino acids (AA) or branched chain amino acid (BCAA) free diets. 42 samples shares translatome
- GSE223687 Effects of astrocytic absence of glucose transporter 1 (GLUT1) on the cortical astrocytic translatome 18 samples shares translatome
- GSE309424 SETDB1/HUSH modulates Xist RNA levels during establishment of X chromosome inactivation 292 samples shares establishment
- GSE206011 Stepwise de novo establishment of inactive X chromosome architecture in early development 262 samples shares establishment
- GSE244496 Establishment of nuclear organization in mouse embryos is regulated by multiple and redundant epigenetic pathways. 183 samples shares establishment
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.