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Hp1bp3 loss links chromatin reorganization to metabolic vulnerability in glioma [ATAC-LOF]

GSE331466 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2026/05/25 Platform GPL21626
Summary
Nuclei were isolated from freshly collected GFP+ tumor tissue and processed for transposase-accessible chromatin profiling using standard ATAC-seq protocols. Tumors were generated through in utero electroporation of triple guide casset targeting Nf1, p53, and Pten (3xCr) plus or minus a guide targeting heterochromatin protein 1 binding partner 3 (Hp1bp3) to assess differences in chromatin accessibility associated with Hp1bp3 deficiency. Sequencing libraries were generated following Tn5 transposition and PCR amplification, then sequenced on an Illumina platform to produce paired-end reads. Raw sequencing data were quality filtered, aligned to the mouse reference genome, and analyzed to identify regions of differential chromatin accessibility between genotypes. These datasets were generated to characterize epigenetic and regulatory landscape alterations associated with Hp1bp3 loss in the context of 3xCr high-grade glioma tumors.
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Direct links to NCBI, no account and no request form: the whole study as GSE331466_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1468191 and SRA study SRP702118. Searching any of these in the dataset finder brings you back here.

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