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Spatial transcriptomics profiling of NDMA-induced liver injury in Mgmt-deficient mice across sex and time

GSE332601 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/05/22 Platform GPL19057
Summary
Spatial transcriptomics (10x Genomics Visium) was performed on fresh-frozen liver tissue (left lobe) from Mgmt-/- mice treated with N-nitrosodimethylamine (NDMA) or saline vehicle during the juvenile period. The study includes male and female mice sacrificed at 10 weeks or 10 months post-birth to characterize spatially resolved transcriptomic changes associated with NDMA-induced liver damage and potential carcinogenesis over time. Mice carried the RaDR+/+; GPT+/+ genotype enabling detection of recombination and mutation events. Tissue sections were processed using the 10x Visium workflow, H&E stained, imaged, permeabilized for spatially barcoded cDNA synthesis, and sequenced on an Illumina NextSeq 500.
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Direct links to NCBI, no account and no request form: the whole study as GSE332601_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1468680 and SRA study SRP702406. Searching any of these in the dataset finder brings you back here.

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