A Fibroblast-Mediated Desmoplastic Reaction Restrains Gastric Cancer via YAP-CTRB1-driven Competitive Fitness

Purpose: To explore the molecular mechanism of CTRB1 boosts host endows cell fitness to outcompete tumor cells, RNA sequencing was performed to analyzeand compare the gene signatures of between MFCSTCL interacted L929 cells (Biotin+) and tumor uninteracted L929 cells (Biotin-). Methods: Tumor interacted or uninteracted L929 cells were sorted. Total mRNA was extracted from tumor interacted (Biotin+) or uninteracted (Biotin-) L929 cells. Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina NovaSeq 6000 platform. Results: Our results revealed differentially expressed genes in Biotin+ L929 cells when compared with Biotin- L929 cells. Further KEGG analysis reveal that expression of CTRB1 influenced several proliferation-related signaling pathways, including Hippo-YAP, WNT, and Notch. Conclusion: Our study present the detailed transcripts analysis of tumor interacted (Biotin+) or uninteracted (Biotin-) L929 cells. Based on RNA-seq transcriptome characterization, indicating that CTRB1 can activate multiple proliferation-related signaling pathways expression to dictate cell fitness during cell competition.