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SETDB1 regulates gene expression in mouse brown adipose tissue and primary brown adipocytes

GSE333495 Mus musculus (mouse) RNA-seq (gene expression) 9 samples Submitted 2026/06/06 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 9 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
9 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
SETDB1 is a histone methyltransferase that catalyzes H3K9me3 and plays critical roles in transcriptional repression. To investigate its function in brown adipose tissue (BAT), we performed RNA-seq across three complementary models: (1) BAT tissue from BAT-specific Setdb1 knockout mice (Setdb1 AKO) vs control mice under thermoneutral conditions; (2) primary brown adipocytes from Setdb1 flox/flox; Rosa26Cre-ERT2 mice treated with 4-hydroxytamoxifen (4-OHT) vs vehicle (ETOH); and (3) primary brown adipocytes transfected with siRNA targeting Setdb1 (siSetdb1) vs non-targeting control siRNA (siNC).
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE333495_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1471191 and SRA study SRP704187. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 9 more — browse all 9 samples with per-sample file links →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.