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The flame retardant triphenyl phosphate induces varying responses in genetically diverse mouse induced pluripotent stem cells

GSE333520 Mus musculus (mouse) RNA-seq (gene expression) 436 samples Submitted 2026/06/01 Platform GPL34290
At a glance

RNA-seq (gene expression) · mouse · 436 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
436 samples: 436 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Genetic variation impacts the biological response to chemical and environmental exposures, contributing to differences in resistance or susceptibility. Forward genetic screens of genetically diverse cell populations can be used to identify the precise genetic variants that drive response variation, but such screens are limited by the lack of available genetically diverse human derived cell panels.
Read the full summary ↓
Cell lines from laboratory mouse genetic reference populations like the Diversity Outbred (DO) population are genetically diverse and powered for genetic mapping but also offer the significant advantage of in vitro / in vivo trait correlation and in vivo validation using genetically matched individuals. These diverse cell panels are especially beneficial for studying early developmental effects of chemical exposures. We created a panel of induced pluripotent stem cells (iPSCs) derived from the Diversity Outbred mouse population to enable genetic analysis of cellular phenotypes. Cells were exposed to triphenyl phosphate (TPHP), an organophosphate flame retardant that has been linked to numerous adverse developmental effects, to identify cellular responses associated with developmental exposure risk. Our analysis characterizes interline variation in gene expression in response to TPHP in over 200 DO iPSC lines by RNA-seq and applied expression quantitative trait loci (eQTL) mapping to identify genomic loci that contribute to variation in gene expression following TPHP exposure.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE333520_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1471224 and SRA study SRP704267. Searching any of these in the dataset finder brings you back here.

Study design
436 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from the first 400 of 436 sample titles: 400 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 396 more — browse all 436 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.