GEO series

Sympathetic Neurons Restrain Pancreatic Cancer Lung Metastatic seeding through NPY-NPY1R Signaling [scRNA-seq]

GSE334205 Mus musculus (mouse) Single-cell RNA-seq 16 samples Submitted 2026/08/06 Platform GPL36595

At a glance

Single-cell RNA-seq · mouse · 16 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism: Mus musculus (mouse)
Samples: 16 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

The sympathetic nervous system regulates physiological homeostasis, but is increasingly recognized as a context dependent modulator of carcinogenesis. Here, we investigate the function of sympathetic innervation on pancreatic ductal adenocarcinoma (PDAC) metastasis. Although sympathetic signaling has been shown to promote primary PDAC growth, its contribution to metastatic progression remains poorly defined.

Read the full summary ↓

Using a pool of barcoded Kras;Trp53-driven mouse PDAC cell lines that allows quantitative analysis of metastatic seeding and growth, we found that sympathetic innervation restrains metastatic seeding but promotes metastatic outgrowth. In vivo pharmacologic screening showed that inhibition of the Neuropeptide Y (NPY) receptor NPY1R phenocopied the effect of sympathetic ablation on metastatic seeding, indicating sympathetic release of NPY restrains metastatic seeding through NPY1R signaling, likely in lung stromal cells including alveolar fibroblasts. Single-cell transcriptional analyses revealed that NPY1R signaling affects alveolar fibroblasts and intermediate gCap endothelial cells, providing a mechanism for restraining vascular permeability and cancer cell seeding. Together, these findings uncover an unexpected metastasis-protective function of sympathetic innervation, distinct from its effect on metastatic growth, and identify a novel sympathetic-stromal signaling axis that regulates the vascular niche during PDAC lung metastasis.

This dataset

Open GSE334205 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE334205_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1474318 and SRA study SRP706475. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 16 more — browse all 16 samples with per-sample file links →

Datasets on a similar topic

GSE313249 A Glucocorticoid-Fas axis controls immune evasion during metastatic seeding [RNAseq_4T1-tdTomato] 30 samples shares metastatic, seeding
GSE299487 LY6D Identifies Persistent Tumor-Initiating Cells Driving Pancreatic Tumorigenesis [scRNA-seq] 12 samples shares pancreatic, scrna-seq
GSE291933 Sensory neurons drive pancreatic cancer progression through glutamatergic cancer-neuron pseudo-synapses 12 samples shares pancreatic, neurons
GSE269888 Oncogenic KRAS-dependent stromal interleukin-33 directs the pancreatic microenvironment to promote tumor growth [scRNA-seq] 11 samples shares pancreatic, scrna-seq
GSE304622 KDM8 is a master regulator for hypoxia-induced metastatic heterogeneity in pancreatic cancer 43 samples shares pancreatic, metastatic
GSE336756 Compartmentalisation of m6A (N6-methyladenosine) methylated RNA in somatic or axonal subcellular locations is dependent on Nerve Growth Factor (NGF) state in sympathetic neurons [Axon-meRIP-seq] 32 samples shares sympathetic, neurons
GSE245821 Metastatic site influences driver gene function in pancreatic cancer [ATAC-seq] 16 samples shares pancreatic, metastatic
GSE308769 eIF4G2-dependent translation restrains pancreatic cancer progression 12 samples shares pancreatic, restrain

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.