Effect of NAT10 knockdown on gene expression in human valve interstitial cells

Calcific aortic valve disease is characterized by osteogenic differentiation and calcification of human valve interstitial cells. We found that NAT10 expression was increased in calcified human aortic valves and that NAT10 positively regulates the calcification of valve interstitial cells. To investigate the downstream regulatory molecules of NAT10 in human valve interstitial cells, RNA-seq was performed in cells with NAT10 knockdown and matched negative controls. Transcriptomic comparison between shNAT10 and shNC groups was used to identify NAT10-regulated genes and pathways potentially involved in valve interstitial cell calcification. These data provide a molecular resource for understanding the role of NAT10 in the regulation of human valve interstitial cell function and calcific aortic valve disease.