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Bulk RNA sequencing reveals endothelial STING-dependent transcriptional programs in murine metabolic dysfunction-associated steatohepatitis

GSE335424 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/06/29 Platform GPL24247
Summary
Liver sinusoidal endothelial cells (LSECs) regulate nutrient flux and immune surveillance within the hepatic niche. Here, we investigate how endothelial-intrinsic cGAS-STING signaling integrates metabolic stress signals to reshape the hepatic niche in metabolic dysfunction-associated steatohepatitis (MASH). We performed bulk RNA sequencing on liver tissues from endothelial cell-specific Sting knockout mice (Stingfl/fl Cdh5-Cre+) and littermate controls (Stingfl/fl Cdh5-Cre-) subjected to a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) for 10 weeks. Gene set enrichment analysis revealed that multiple cancer-associated and fibrogenic signaling pathways were enriched in control livers but markedly suppressed in StingΔEC livers. Conversely, pathways related to T cell regulation and metabolism were preferentially enriched in StingΔEC livers, indicating that endothelial-intrinsic STING signaling drives MASH progression by reshaping T cell-mediated immune responses.
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Also filed as BioProject PRJNA1478524 and SRA study SRP709638. Searching any of these in the dataset finder brings you back here.

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