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Single-cell RNA sequencing of ovarian CD45+ immune cells from gonadotropin-treated and untreated mice

GSE336281 Mus musculus Expression profiling by high throughput sequencing; Other 4 samples Submitted 2026/06/24 Platform GPL17021
Summary
CD45+ immune cells were isolated from ovaries of adult female C57BL/6J mice treated with gonadotropin (n=5) or saline control (n=5). Individual mice were labeled with TotalSeq-B hashtag antibodies, pooled by treatment group, and subjected to 10x Genomics Chromium single-cell RNA sequencing. Hashtag oligonucleotide libraries were used for sample demultiplexing. The dataset was generated to characterize ovarian immune cell populations and their response to gonadotropin-induced hormonal stimulation.
Published in
Single-cell analysis of ovarian immune cells reveals dynamic changes in NK and B-cell populations after ovarian stimulation
Brodeur TY, Lanser TB, Salter L et al. · Journal of immunology (Baltimore, Md. : 1950) 2026 · PMID 42467591 · doi:10.1093/jimmun/vkag153
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Direct links to NCBI, no account and no request form: the whole study as GSE336281_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1481202 and SRA study SRP711878. Searching any of these in the dataset finder brings you back here.

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