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iMSC-Derived Extracellular Vesicles Attenuate Cisplatin-Induced Renal Tubulointerstitial Fibrosis

GSE341828 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/08/01 Platform GPL34328
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × Normal control kidney, vs 3 × Cisplatin plus PBS kidney, vs 3 × Cisplatin plus iMSC-EVs 200 ug kidney, vs 3 × Cisplatin plus iMSC-EVs 400 ug kidney,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Background: Cisplatin is a widely used chemotherapeutic agent for various solid tumors. However, its use is often limited by nephrotoxicity, which can lead to acute kidney injury and, with repeated exposure, cumulative tubular damage and chronic renal dysfunction. Induced mesenchymal stem cell-derived extracellular vesicles (iMSC-EVs) have emerged as a promising cell-free therapeutic approach because of their anti-inflammatory, anti-apoptotic, and regenerative properties.
Read the full summary ↓
The therapeutic efficacy of iMSC-EVs in cumulative cisplatin nephrotoxicity remains unclear. Methods: Male C57BL/6 mice were subjected to renal injury by intraperitoneal cisplatin administration at 9 mg/kg once weekly for five cycles. After the final cisplatin injection, mice received intravenous PBS or iMSC-EVs at doses of 200 or 400 μg every 3 days for five doses. Renal injury, fibrosis, inflammation, cell-death signaling, oxidative stress, and peritubular capillary loss were evaluated by histology, immunoblotting, immunostaining, and quantitative polymerase chain reaction. RNA sequencing of renal tissue was performed to identify pathways regulated by iMSC-EV treatment. Results: iMSC-EVs ameliorated repeated cisplatin-induced renal injury in a dose-associated manner, with the 400 μg group showing more pronounced therapeutic effects. iMSC-EV treatment reduced collagen deposition, tubular damage, immune-cell infiltration, and peritubular capillary loss. Fibrosis-related markers and inflammatory mediators were suppressed by iMSC-EV. RNA-sequencing of renal tissues revealed that iMSC-EV treatment modulated and partially reversed RLDC-induced transcriptional alterations, particularly those associated with inflammatory, immune, and extracellular matrix-related pathways. Conclusion: iMSC-EVs mitigated key pathological features of repeated cisplatin-induced kidney injury, with the most consistent effects observed at the 400 μg dose. These findings support iMSC-EVs as a promising cell-free therapeutic strategy for tubulointerstitial fibrosis induced by cumulative cisplatin nephrotoxicity.
This dataset
Open GSE341828 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE341828_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1504240 and SRA study SRP722448. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
3 × Normal control kidney, vs 3 × Cisplatin plus PBS kidney, vs 3 × Cisplatin plus iMSC-EVs 200 ug kidney, vs 3 × Cisplatin plus iMSC-EVs 400 ug kidney,

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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