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A configurable AP-1 network governs cell-state heterogeneity and adaptive plasticity in melanoma cells

GSE342614 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing 8 samples Submitted 2026/08/06 Platform GPL30173
Summary
AP-1 transcription factors have been implicated in cellular plasticity, differentiation-state heterogeneity, and phenotype switching in response to cancer therapies. To understand the transcriptional consequences of AP-1 perturbations at a single-cell level, we depleted JUND and JUNB expression in COLO858 cells using shRNA-mediated knockdown (JUNDKD and JUNBKD, respectively). We then performed single-cell multiome (RNA + ATAC) sequencing in these cells and compared with COLO858 cells expressing non-targeting shRNA control.
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Also filed as BioProject PRJNA1508657 and SRA study SRP724663. Searching any of these in the dataset finder brings you back here.

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