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Ikaros affects multiple lineages in hematopoietic development [RNA-Seq]

GSE39069 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2026/05/30 Platform GPL13112
Summary
Ikaros is a zinc finger transcription factor critical for proper hematopoietic development and tumor suppression in the lymphoid lineage. Here, we use the newly generated Ik-ZnF1-/- and Ik-ZnF4-/- mutant mice to study hematopoietic development in the BM. As an initial approach to study early hematopoietic development as well as non-lymphoid lineages, RNA-Seq was performed on "Hardy Fraction A" cells, without lineage depletion (B220+IgM-CD43+CD24-BP-1-), of wt and Ik-ZnF1-/- mice (two biological replicates of each). Ik-ZnF4-/- mice have extremely few cells in the "Hardy fraction A" gate, and only one RNA-Seq experiment was performed on this cell population, with strongly reduced reads/genome coverage.
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Direct links to NCBI, no account and no request form: the whole study as GSE39069_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 5 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA169876 and SRA study SRP013993. Searching any of these in the dataset finder brings you back here.

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