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Genome-wide quantitative enhancer activity maps identified by STARR-seq

GSE40739 Homo sapiens; Drosophila melanogaster Expression profiling by high throughput sequencing; Other; Genome binding/occupancy profiling by high throughput sequencing 18 samples Submitted 2013/01/17 Platform GPL11154Platform GPL11203Platform GPL13304
Summary
Genomic enhancers are important regulators of gene expression, but their identification is a challenge and methods depend on indirect measures of activity. We developed a method termed STARR-seq to directly and quantitatively assess enhancer activity for millions of candidates from arbitrary sources of DNA, enabling screens across entire genomes. When applied to the Drosophila genome, STARR-seq identifies thousands of cell type-specific enhancers across a broad continuum of strengths, linking differential gene expression to differences in enhancer activity and creating a genome-wide quantitative enhancer map. This map reveals the highly complex regulation of transcription, with several independent enhancers for both developmental regulators and ubiquitously expressed genes. STARR-seq can be used to identify and quantitate enhancer activity in other eukaryotes, including human.
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Direct links to NCBI, no account and no request form: the whole study as GSE40739_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 18 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA175267 and SRA study SRP015758. Searching any of these in the dataset finder brings you back here.

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