GEO series
Macrophage Microvesicles Induce Macrophage Differentiation and miR-223 Transfer
At a glance
Expression microarray · human · 24 samples
- What it is
- Expression microarrayGene activity read on a chip (the older technology that RNA-seq replaced).
- Organism
- Homo sapiens (human)
- Samples
- 24 samples: 8 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed expression table (series matrix) and the array files, from NCBI.
Summary (written by the submitters)
Microvesicles (MV) are small membrane-bound particles comprised of exosomes and various sized extracellular vesicles. These are released by a number of cell types. Microvesicles have a variety of cellular functions from communication to mediating growth and differentiation. Microvesicles contain proteins and nucleic acids. Previously, we showed that plasma microvesicles contain microRNAs (miRNAs).
Read the full summary ↓
Based on our previous report, the majority of peripheral blood microvesicles are derived from platelets while mononuclear phagocytes, including macrophages, are the second most abundant population. Here, we characterized macrophage-derived microvesicles and whether they influenced the differentiation of naïve monocytes. We also identified the miRNA content of the macrophage-derived microvesicles. We found that RNA molecules contained in the macrophage-derived microvesicles were transported to target cells, including monocytes, endothelial cells, epithelial cells and fibroblasts. Furthermore, we found that miR-223 was transported to target cells and was functionally active. Based on our observations, we hypothesize that microvesicles bind to and activate target cells. Furthermore, we find that microvesicles induce the differentiation of macrophages. Thus, defining key components of this response may identify novel targets to regulate host defense and inflammation. We used GeneChip microarrays to examine changes in gene expression induced by MV in primary monocyte-derived macrophages (MDM) and in THP1 cells, and compare this to cells treated with GM-CSF and PMA, respectively.
This dataset
Download · which file do I need?
Direct links to NCBI, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
1 · Just have a look
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
2 · Re-analyse it
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need.
3 · The original raw files
Whole study · RAW.tar
Everything the authors deposited as one archive (GSE41889_RAW.tar), including the raw array or instrument files. Can be large; the picker in step 2 takes just the samples you need.
What the files usually look like: For arrays the series matrix is normally the normalised expression table you want; the raw array files (CEL and similar) are in the RAW.tar.
Also filed as BioProject PRJNA178399. Searching any of these in the dataset finder brings you back here.
Study design
8 conditions, mostly in triplicate
Freshly isolated monocytes used as MV donors, ×3
GM-CSF-treated macrophages used as MV donors, ×3
Freshly isolated monocytes used as MV recipie… ×3
Freshly isolated monocyte incubated 24 h with… ×3
THP1+DMSO used as MV donors, ×3
THP1 incubated 24 h with MV from THP1-DMSO do… ×3
THP1+PMA used as MV donors, ×3
THP1 incubated 24 h with MV from THP1-PMA don… ×3
Supports a case/control comparison: 6 samples read as cases, 6 as controls.
How we read this: 8 replicated groups read from 24 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
Samples in this study
- GSM1026969 Freshly isolated monocytes used as MV donors, repl. 1 Freshly isolated monocy…
- GSM1026970 Freshly isolated monocytes used as MV donors, repl. 2 Freshly isolated monocy…
- GSM1026971 Freshly isolated monocytes used as MV donors, repl. 3 Freshly isolated monocy…
- GSM1026972 GM-CSF-treated macrophages used as MV donors, repl. 1 GM-CSF-treated macropha…
- GSM1026973 GM-CSF-treated macrophages used as MV donors, repl. 2 GM-CSF-treated macropha…
- GSM1026974 GM-CSF-treated macrophages used as MV donors, repl. 3 GM-CSF-treated macropha…
- GSM1026975 Freshly isolated monocytes used as MV recipients, repl. 1 Freshly isolated monocy…
- GSM1026976 Freshly isolated monocytes used as MV recipients, repl. 2 Freshly isolated monocy…
- GSM1026977 Freshly isolated monocytes used as MV recipients, repl. 3 Freshly isolated monocy…
- GSM1026978 Freshly isolated monocyte incubated 24 h with MV from other GM-CSF-treated MDM, repl. 1 Freshly isolated monocy…
- GSM1026979 Freshly isolated monocyte incubated 24 h with MV from other GM-CSF-treated MDM, repl. 2 Freshly isolated monocy…
- GSM1026980 Freshly isolated monocyte incubated 24 h with MV from other GM-CSF-treated MDM, repl. 3 Freshly isolated monocy…
- GSM1026981 THP1+DMSO used as MV donors, repl. 1 THP1+DMSO used as MV do…
- GSM1026982 THP1+DMSO used as MV donors, repl. 2 THP1+DMSO used as MV do…
- GSM1026983 THP1+DMSO used as MV donors, repl. 3 THP1+DMSO used as MV do…
- GSM1026984 THP1 incubated 24 h with MV from THP1-DMSO donors, repl. 1 THP1 incubated 24 h wit…
- GSM1026985 THP1 incubated 24 h with MV from THP1-DMSO donors, repl. 2 THP1 incubated 24 h wit…
- GSM1026986 THP1 incubated 24 h with MV from THP1-DMSO donors, repl. 3 THP1 incubated 24 h wit…
- GSM1026987 THP1+PMA used as MV donors, repl. 1 THP1+PMA used as MV don…
- GSM1026988 THP1+PMA used as MV donors, repl. 2 THP1+PMA used as MV don…
- GSM1026989 THP1+PMA used as MV donors, repl. 3 THP1+PMA used as MV don…
- GSM1026990 THP1 incubated 24 h with MV from THP1-PMA donors, repl. 1 THP1 incubated 24 h wit…
- GSM1026991 THP1 incubated 24 h with MV from THP1-PMA donors, repl. 2 THP1 incubated 24 h wit…
- GSM1026992 THP1 incubated 24 h with MV from THP1-PMA donors, repl. 3 THP1 incubated 24 h wit…
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.