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Xist exploits three-dimensional chromosome architecture to spread across the X-chromosome

GSE46918 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 37 samples Submitted 2013/05/30 Platform GPL13112
Summary
Many large noncoding RNAs (lncRNAs) regulate chromatin, but the mechanisms by which they localize to genomic targets remain unexplored. Here we investigate the localization mechanisms of Xist during X-chromosome inactivation (XCI), a paradigm of lncRNA-mediated chromatin regulation. During the maintenance of XCI, Xist binds broadly across the X-chromosome. During initiation of XCI, Xist initially transfers to distal regions across the X-chromosome that are not defined by specific sequences. Instead, Xist identifies these regions by exploiting the three-dimensional conformation of the X-chromosome. Xist initially accumulates on the periphery of actively transcribed regions and requires its silencing domain to spread across active regions. This suggests a model where Xist coats the entire X-chromosome by searching in three dimensions, modifying chromosome structure, and spreading to newly accessible locations.
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Also filed as BioProject PRJNA203042 and SRA study SRP022711. Searching any of these in the dataset finder brings you back here.

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