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Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position

GSE47753 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 13 samples Submitted 2013/10/01 Platform GPL11154
Summary
We describe an assay for transposase-accessible chromatin using sequencing (ATAC-seq), based on direct in vitro transposition of sequencing adaptors into native chromatin, as a rapid and sensitive method for integrative epigenomic analysis. ATAC-seq captures open chromatin sites using a simple two-step protocol with 500–50,000 cells and reveals the interplay between genomic locations of open chromatin, DNA-binding proteins, individual nucleosomes and chromatin compaction at nucleotide resolution. We discovered classes of DNA-binding factors that strictly avoided, could tolerate or tended to overlap with the nucleosome. Using ATAC-seq maps of human CD4+ T cells from a proband obtained on consecutive days, we demonstrated the feasibility of analyzing an individual’s epigenome on a timescale compatible with clinical decision-making.
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Direct links to NCBI, no account and no request form: the whole study as GSE47753_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 13 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA207663 and SRA study SRP024293. Searching any of these in the dataset finder brings you back here.

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