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RNA-RNA interactions enable specific targeting of noncoding RNAs to nascent pre-mRNAs and chromatin sites

GSE55914 Mus musculus Genome binding/occupancy profiling by high throughput sequencing; Other 46 samples Submitted 2014/09/01 Platform GPL17021
Summary
We developed a general method based on RNA Antisense Purification (RAP) to identify the intermolecular RNA-RNA interactions of a target RNA (RAP-RNA). RAP-RNA identifies endogenous RNA-RNA complexes through in vivo crosslinking, RNA capture with antisense oligonucleotides, and high-throughput RNA sequencing. This approach provides a systematic view of other RNAs that interact with a target RNA, and furthermore can distinguish between direct and indirect RNA-RNA interactions through the use of crosslinking reagents with different reactivities with proteins and nucleic acids. We applied this method to numerous small and large noncoding RNAs, including U1 snRNA, Malat1 lncRNA, Xist lncRNA, U3 snoRNA, U17/Snora73a snoRNA and U12 snRNA.
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Direct links to NCBI, no account and no request form: the whole study as GSE55914_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 46 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA241313 and SRA study SRP040132. Searching any of these in the dataset finder brings you back here.

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