← BioTransfer GEO Dataset Finder
GEO series

Single-Cell Whole-Genome Bisulfite Sequencing for Analysis of Epigenetic Heterogeneity

GSE56879 Mus musculus Methylation profiling by high throughput sequencing 56 samples Submitted 2014/07/18 Platform GPL13112
Summary
We report a single-cell whole-genome bisulfite sequencing method (scBS-Seq) capable of accurately measuring DNA methylation at up to 36% of CpGs. We observed that ESCs grown in serum/LIF or 2i/LIF both display epigenetic heterogeneity, with “2i-like” cells present in serum cultures. In silico integration of 12 individual MII oocytes datasets  recapitulates the whole DNA methylome, making scBS-Seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE56879_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 56 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA244869 and SRA study SRP041257. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 56 more — browse all 56 samples with per-sample file links →

Similar datasets

Search all mouse methylation datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.