← BioTransfer GEO Dataset Finder
GEO series

An Oncogenic Super-Enhancer Formed through Somatic Mutation of a Noncoding Intergenic Element

GSE59657 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 20 samples Submitted 2014/11/13 Platform GPL16791Platform GPL11154Platform GPL9115
Summary
In many cancers, critical oncogenes are driven from large regulatory elements, called super-enhancers, which recruit much of the cell’s transcriptional apparatus and are defined by extensive H3K27 acetylation. We found that in T-cell acute lymphoblastic leukemia (T-ALL), somatic heterozygous mutations introduce MYB binding motifs in a precise noncoding site, which nucleate a super-enhancer upstream of the TAL1 oncogene. Further analysis of genome-wide binding identified MYB and its histone acetylase binding partner CBP as core components of the TAL1 complex and of the TAL1-mediated feed-forward auto-regulatory loop that drives T-ALL. Furthermore, MYB and CBP occupy endogenous MYB binding sites in the majority of super-enhancer sites found in T-ALL cells. Thus, our study reveals a new mechanism for the generation of super-enhancers in malignant cells involving the introduction of somatic indel mutations within non-coding sequences, which introduce aberrant binding sites for the MYB master transcription factor.
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE59657_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 20 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA255872 and SRA study SRP044701. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 20 more — browse all 20 samples with per-sample file links →

Similar datasets

Search all human ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.