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Human Pancreatic CTCs Express the ECM Protein SPARC

GSE60407 Homo sapiens Expression profiling by high throughput sequencing 7 samples Submitted 2014/09/22 Platform GPL16288
Summary
Circulating Tumor Cells (CTCs) are shed from primary tumors into the bloodstream, mediating the hematogenous spread of cancer to distant organs. To determine the relevance of ECM protein expression to human disease, CTCs were isolated from the blood of metastatic PDAC patients and subjected to single cell RNA-sequencing. Analysis of 7 pancreatic CTCs from 3 patients revealed that the majority expressed keratins defining their epithelial origin. A total of 13 of 60 extracellular protein genes enriched in mouse CTCs (see GEO GSE51372) were expressed at high levels (>100 rpm) in at least one human pancreatic CTC. Human SPARC was the only gene found at high levels in all human pancreatic CTCs.
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Direct links to NCBI, no account and no request form: the whole study as GSE60407_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA258196 and SRA study SRP045493. Searching any of these in the dataset finder brings you back here.

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