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m6A mRNA Methylation Facilitates Resolution of Naïve Pluripotency Towards Differentiation (RNA-Seq)

GSE61997 Mus musculus Expression profiling by high throughput sequencing 11 samples Submitted 2014/11/02 Platform GPL17021
Summary
Naïve and primed pluripotent states retain distinct molecular properties, yet limited knowledge exists on how their state transitions are regulated. Here, we identify Mettl3, an N6-methyladenosine (m6A) transferase, as a regulator for terminating murine naïve pluripotency. Mettl3 knockout pre-implantation epiblasts and naïve embryonic stem cells (ESCs) are depleted for m6A in mRNAs and yet, are viable. However, they fail to adequately terminate their naïve state, and subsequently undergo aberrant and restricted lineage priming at the post-implantation stage, leading to early embryonic lethality. m6A predominantly and directly reduces mRNA stability, including that of key naïve pluripotency promoting transcripts. This study highlights a critical role for an mRNA epigenetic modification in vivo, and identifies regulatory modules that functionally influence naïve and primed pluripotency in an opposing manner.
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Direct links to NCBI, no account and no request form: the whole study as GSE61997_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 11 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA262910 and SRA study SRP048598. Searching any of these in the dataset finder brings you back here.

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