GEO series

Immune and HCT116 RNA mixtures

GSE64385 Homo sapiens Expression profiling by array 12 samples Submitted 2016/02/18 Platform GPL570

Summary

These experiments were designed as a benchmark tool for deconvolution methods. 5 immune cell populations were sorted from 3 healthy donors' peripheral bloods. Peripheral Blood Mononuclear Cells (PBCMs) and PolymorphoNuclear Cells (PMN) were separated using gradient centrifugation. T cells (DAPI-/CD3+/CD14-/CD19-/CD56-), monocytes (DAPI-/CD3-/CD14+/CD19-/CD56-), B cells (DAPI-/CD3-/CD14-/CD19+/CD56-) and NK cells (DAPI-/CD3-/CD14-/CD19-/CD56+) were FACS-sorted from PBMCs and neutrophils (DAPI-/CD66b+/CD19-/CD3-/CD56-/CD14-) were sorted from PMNs. RNA was extracted from the purified cell population, as well as from the HCT116 colon cancer cell line. RNAs from pure populations were then mixed in various proportions.

This dataset

Open GSE64385 in finder → Find more human microarray datasets →

Download

Direct links to NCBI, no account and no request form: the whole study as GSE64385_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 12 samples.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗

Also filed as BioProject PRJNA270868. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 27765066) ↗

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 12 more — browse all 12 samples with per-sample file links →

Similar datasets

GSE11121 The humoral immune system has a key prognostic impact in node-negative breast cancer 200 samples
GSE7965 Blood and Adipose tissue samples 1722 samples
GSE194040 I-SPY2-990 mRNA/RPPA Data Resource: mRNA component 988 samples
GSE37069 Gene response to major burn injuries 590 samples
GSE33315 Discovery of novel recurrent mutations and rearrangements in early T-cell precursor acute lymphoblastic leukaemia by whole genome sequencing 575 samples
GSE5258 Connectivity Map dataset (build01) 564 samples
GSE76009 A 17-Gene Stemness Score for Rapid Identification of High-Risk AML Patients 534 samples
GSE25066 Genomic predictor of response and survival following neoadjuvant taxane-anthracycline chemotherapy in breast cancer 508 samples

Search all human microarray datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.