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Efficient and quantitative high-throughput tRNA sequencing

GSE66550 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2015/07/01 Platform GPL15433
Summary
Despite its biological importance, transfer RNA (tRNA) could not be adequately sequenced due to the abundant presence of post-transcriptional modifications and extensive structure that interfere with cDNA synthesis and adapter ligation. We achieve efficient and quantitative tRNA sequencing by removing base methylations using engineered demethylases and using a highly processive thermo-stable reverse transcriptase without the need for adapter ligation (DMTRT-tRNA-seq). Our method should be applicable for biological investigations of tRNA in all organisms.
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Also filed as BioProject PRJNA277309 and SRA study SRP055858. Searching any of these in the dataset finder brings you back here.

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