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Evidence for proliferation and synaptogenesis impairments in neural cells derived from idiopathic autistic patients

GSE67528 Homo sapiens Expression profiling by high throughput sequencing 83 samples Submitted 2016/07/10 Platform GPL11154
Summary
Reprogramming of human somatic cells to a pluripotent state (induced pluripotent stem cells or iPSC) has provided an exciting opportunity to produce relevant cellular models of human complex neurogenetic diseases. Here we show the generation and characterization of iPSC lines from 8 sporadic ASD patients with early brain overgrowth and 5 age/gender-matched control lines. These cells were used to derive neural progenitor cells and neurons in culture. We reveal molecular and phenotypic evidence that both cell proliferation and synaptogenesis are affected in this model system. Specifically, we detected altered cell cycle and altered levels of excitatory and inhibitory markers in neural cells at early stages of differentiation. This work demonstrates that in a heterogeneous condition such as ASD, selection of subjects based on phenotype can improve power to detect biologically relevant pathway disruption that may help guide development of novel therapies.
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Direct links to NCBI, no account and no request form: the whole study as GSE67528_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 83 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA280163 and SRA study SRP056822. Searching any of these in the dataset finder brings you back here.

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