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Multiplex Single Cell Profiling of Chromatin Accessibility by Combinatorial Cellular Indexing

GSE68103 Mus musculus; Homo sapiens Other; Expression profiling by high throughput sequencing 12 samples Submitted 2015/05/07 Platform GPL15520Platform GPL19969Platform GPL18573
Summary
Technical advances have enabled the collection of genome and transcriptome data sets with single-cell resolution. However, single-cell characterization of the epigenome has remained challenging. Furthermore, because cells must be physically separated prior to biochemical processing, conventional single-cell preparatory methods scale linearly. We applied combinatorial cellular indexing to measure chromatin accessibility in thousands of single cells per assay, circumventing the need for compartmentalization of individual cells. We report chromatin accessibility profiles from over 15,000 single cells and use these data to cluster cells on the basis of chromatin accessibility landscapes. We identify modules of coordinately regulated chromatin accessibility at the level of single cells both between and within cell types, with a scalable method that may accelerate progress toward a human cell atlas. This SuperSeries is composed of the SubSeries listed below.
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Direct links to NCBI, no account and no request form: the whole study as GSE68103_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 12 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA281742 and SRA study SRP057508. Searching any of these in the dataset finder brings you back here.

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