GEO series

Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle micro RNAs

GSE71241 Homo sapiens (human) Non-coding RNA profiling 18 samples Submitted 2015/08/17 Platform GPL18743

At a glance

Non-coding RNA profiling · human · 18 samples

What it is: Non-coding RNA profilingLevels of RNAs that do not code for proteins, such as miRNA or lncRNA.
Organism: Homo sapiens (human)
Samples: 18 samples
Data you can get: A processed expression table (series matrix) and the array files, from NCBI.

Summary (written by the submitters)

Background: Mesenchymal stem cells (MSCs) and macrophages are fundamental components of the stem cell niche and function coordinately to regulate hematopoietic stem cell self-renewal and mobilization. Recent studies indicate that mitophagy and healthy mitochondrial function are critical to the survival of stem cells but how these processes are regulated in MSCs is unknown.

Read the full summary ↓

Here we show that MSCs manage intracellular oxidative stress by targeting depolarized mitochondria to the plasma membrane via arrestin domain-containing protein 1-mediated microvesicles , which are then engulfed and re-utilized via a process involving fusion by macrophages resulting in enhanced bio-energetics. Furthermore, we show that MSCs simultaneously shed miRNA-containing exosomes that inhibit macrophage activation by suppressing Toll-like receptor (TLR) signaling, thereby de-sensitizing macrophages to the ingested mitochondria. Collectively, these studies mechanistically link mitophagy and MSC survival with macrophage function, thereby providing a physiologically relevant context for the innate immuno-modulatory activity of MSCs.

This dataset

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Download · which file do I need?

Direct links to NCBI, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need.

All supplementary files ↗

3 · The original raw files

Whole study · RAW.tar

Everything the authors deposited as one archive (GSE71241_RAW.tar), including the raw array or instrument files. Can be large; the picker in step 2 takes just the samples you need.

Whole study · RAW.tar ↓

Also filed as BioProject PRJNA290656. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 26442449) ↗

Samples in this study

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+ 18 more — browse all 18 samples with per-sample file links →

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