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Single cell RNA sequencing of primary breast cancer.

GSE75688 Homo sapiens Expression profiling by high throughput sequencing 563 samples Submitted 2016/12/09 Platform GPL16791
Summary
We performed single cell RNA sequencing (RNA-seq) for 549 primary breast cancer cells and lymph node metastases from 11 patients with distinct molecular subtypes (BC01-BC02, estrogen receptor positive (ER+); BC03, double positive (ER+ and HER2+); BC03LN, lymph node metastasis of BC03; BC04-BC06, human epidermal growth factor receptor 2 positive (HER2+); BC07-BC11, triple-negative breast cancer (TNBC); BC07LN, lymph node metastasis of BC07) and matched bulk tumors. We separated these single cells into epithelial tumor and tumor-infiltrating immune cells using inferred CNVs from RNA-seq. The refined single cell profiles for the tumor and immune cells provide key expression signatures of breast cancer and the surrounding microenvironment.
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Direct links to NCBI, no account and no request form: the whole study as GSE75688_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 563 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA305054 and SRA study SRP066982. Searching any of these in the dataset finder brings you back here.

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