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ChIP-exo of human KRAB-ZNFs transduced in HEK 293T cells and KAP1 in hES H1 cells

GSE78099 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 237 samples Submitted 2017/03/06 Platform GPL16791
Summary
Encoded in the hundreds by the human genome, KRAB-containing zinc finger proteins (KRAB-ZFPs) constitute a rapidly evolving family of transcription factors with largely undefined functions. Here, by a combination of phylogenetic and genomic approaches, we retrace the evolutionary history of KRAB-ZFP genes and define the genomic targets of their human products. Through in silico analysis of 207 vertebrate genomes and chromatin immunoprecipitation / deep sequencing characterization of 257 human KRAB-ZFPs, we identify the root of the family in an early Devonian ancestor of tetrapods, describe its diversity amongst these species, and reveal that the majority of its human members primarily recognize transposable elements. Furthermore, by dissecting the timeline and modalities of interactions between human KRAB-ZFPs and their targets, we provide evidence strongly suggesting that these proteins, rather than just engaged in an evolutionary arms race against transposable elements, exploit these invaders as regulatory platforms for the benefit of the host.
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Direct links to NCBI, no account and no request form: the whole study as GSE78099_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 237 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA312613 and SRA study SRP070561. Searching any of these in the dataset finder brings you back here.

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