GEO series

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells [human]

GSE78115 Homo sapiens (human) Expression microarray 21 samples Submitted 2017/03/02 Platform GPL16686

At a glance

Expression microarray · human · 21 samples

What it is: Expression microarrayGene activity read on a chip (the older technology that RNA-seq replaced).
Organism: Homo sapiens (human)
Samples: 21 samples
Data you can get: A processed expression table (series matrix) and the array files, from NCBI.

Summary (written by the submitters)

Microglia are myeloid-lineage inflammatory cells that activate innate and adaptive immune responses within the central nervous system (CNS). They also have important roles in CNS development and homeostasis and can modulate the course of a variety of CNS diseases. We report here the sequential differentiation of murine induced pluripotent stem cells (iPSC) into hematopoietic progenitor-like cells and then into cells with a phenotype and gene expression profile resembling that of primary brain-isolated microglia.

Read the full summary ↓

Functionally, the iPSC-derived microglia (iPS-MG) produce inflammatory cytokines and reactive oxygen species, demonstrate phagocytic activity and migrate to areas of pathology within the brain following intracranial injection. The ability to readily generate iPS-MG in vitro will facilitate the study of normal and disease-specific microglia and be useful for the development of microglia-based treatments for CNS diseases.

This dataset

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Download · which file do I need?

Direct links to NCBI, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need.

All supplementary files ↗

3 · The original raw files

Whole study · RAW.tar

Everything the authors deposited as one archive (GSE78115_RAW.tar), including the raw array or instrument files. Can be large; the picker in step 2 takes just the samples you need.

Whole study · RAW.tar ↓

What the files usually look like: For arrays the series matrix is normally the normalised expression table you want; the raw array files (CEL and similar) are in the RAW.tar.

Also filed as BioProject PRJNA312652. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 28253233) ↗

Samples in this study

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+ 21 more — browse all 21 samples with per-sample file links →

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