GEO series

Autophagy maintains metabolism and functional activity of a subset of aged hematopoietic stem cells [RRBS]

GSE81720 Mus musculus Methylation profiling by high throughput sequencing 8 samples Submitted 2017/03/03 Platform GPL17021

Summary

Autophagy is critical for protecting HSCs from metabolic stress. Here, we used a genetic approach to inactivate autophagy in adult HSCs by deleting the Atg12 gene. We show that loss of autophagy causes accumulation of mitochondria and an oxidative phosphorylation (OXPHOS)-activated metabolic state, which drives accelerated myeloid differentiation likely through epigenetic deregulations rather than transcriptional changes, and impairs HSC self-renewal activity and regenerative potential. To determine how loss of autophagy affects DNA methylation, we conducted enhanced reduce representation bisulfite sequencing (ERRBS) of purified control and Atg12 conditional knockout HSCs.

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Direct links to NCBI, no account and no request form: the whole study as GSE81720_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA322387 and SRA study SRP075503. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 28241143) ↗

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