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Next-generation sequencing and quantitative analysis of wild type and p110gamma -/- tumors and tumor-associated macrophages

GSE84535 Mus musculus Expression profiling by high throughput sequencing 14 samples Submitted 2016/08/25 Platform GPL13112
Summary
Purpose: The goals of this study were to identify quantitative gene expression differences between whole tumor and tumor-associated macrophages (TAMs) derived from Lewis lung carcinoma (LLC) tumors grown in wild type and PI3Kinase-gamma-null mice. Methods: mRNA profiles of whole tumor or tumor-associated macrophages (CD11b+Gr1- cells) from wild type (WT) or PI3Kinase-gamma-knockout (p110g-/-) mice were generated by single deep read sequencing, in triplicate or quadruplicate, using Illumina HiSeq 2000. The sequence reads that passed quality filters were aligned to mouse transcriptome using the bowtie2 aligner. Gene-level summaries were normalized and analyzed for differential expression using DESeq.
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Direct links to NCBI, no account and no request form: the whole study as GSE84535_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 14 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA329549 and SRA study SRP078862. Searching any of these in the dataset finder brings you back here.

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