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Transcriptomic profiles of intestinal stem cells cultured in natural and synthetic three-dimensional matrices

GSE85391 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2016/11/01 Platform GPL17021
Summary
We used RNA-seq to define the gene expression profiles of intestinal stem cells (ISCs) expanded in Matrigel, degradable poly(ethylene) glycol (PEG) and non-degradable PEG matrices. Comparison of mRNA profiles between ISCs grown in Matrigel and non-degradable PEG show no major differences in expression of gene related to stemness, proliferation and signaling via the Wnt and Notch pathways. These results also show that ISC cultured in degradable PEG matrices upregulate stress- and inflammation-related genes compared with cells expanded in non-degradable PEG matrices.
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Also filed as BioProject PRJNA338315 and SRA study SRP081167. Searching any of these in the dataset finder brings you back here.

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