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Seq-Well: A portable, low-cost platform for single-cell RNA-seq of low-input samples

GSE92495 Mus musculus; Homo sapiens Expression profiling by high throughput sequencing 7 samples Submitted 2017/02/17 Platform GPL18573Platform GPL19415
Summary
Single-cell RNA -seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA -seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We characterize Seq-Well extensively and use it to profile thousands of primary human macrophages exposed to tuberculosis.
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Direct links to NCBI, no account and no request form: the whole study as GSE92495_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA374551 and SRA study SRP099447. Searching any of these in the dataset finder brings you back here.

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