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Pooled CRISPR screening with single-cell transcriptome read-out

GSE92872 Homo sapiens; Mus musculus Expression profiling by high throughput sequencing 100 samples Submitted 2017/01/18 Platform GPL19969Platform GPL20301Platform GPL18573
Summary
We combined CRISPR genome editing with single-cell RNA sequencing to assess complex phenotypes in pooled cellular screens. Our method for CRISPR droplet sequencing (CROP-seq) comprises four key components: a gRNA vector that makes individual gRNAs detectable in single-cell transcriptomes, a high-throughput assay for single-cell RNA-seq, a computational pipeline for assigning single-cell transcriptomes to gRNAs, and a bioinformatic method for analyzing and interpreting gRNA-induced transcriptional profiles. CROP-seq allowed us to link gRNA expression to the associated transcriptome responses in thousands of single cells using a straightforward and broadly applicable screening workflow. Additional information are available from the CROP-seq website http://crop-seq.computational-epigenetics.org
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Also filed as BioProject PRJNA358686 and SRA study SRP095602. Searching any of these in the dataset finder brings you back here.

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