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Single-base resolution methylome of 5-Methylcytosine in human and mouse transcriptome

GSE93749 Mus musculus; Homo sapiens Methylation profiling by high throughput sequencing 22 samples Submitted 2017/04/19 Platform GPL17021Platform GPL16791
Summary
mRNAs of siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement. mRNAs were fragmented to ~200 nt and treated by Sodium bisulfite solution (pH 5.1) containing Hydroquinone. Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA) and performed sequencing on HiSeq2500 (Illumina) in pair-end mode, creating reads with a length of 125 bp. Sequencing chemistry v4 (Illumina) was used and every sample was sequenced at one lane.
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Direct links to NCBI, no account and no request form: the whole study as GSE93749_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 22 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA362514 and SRA study SRP097125. Searching any of these in the dataset finder brings you back here.

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