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Self-organized cerebral organoids with human specific features predict effective drugs to combat Zika virus infection [RNA-seq]

GSE97919 Homo sapiens Expression profiling by high throughput sequencing 12 samples Submitted 2017/10/11 Platform GPL21290
Summary
The human cerebral cortex possess distinct structural and functional features that are not found in the lower species traditionally used to model brain development and disease. Accordingly, considerable attention has been placed on the development of methods to direct pluripotency stem cells to form human brain-like structures termed organoids. However, many organoid differentiation protocols are inefficient and display marked variability in their ability to recapitulate the three-dimensional architecture and course of neurogenesis in the developing human brain. Here, we report optimized organoid culture methods that efficiently and reliably produce cortical and basal ganglia structures similar to those in the human fetal brain in vivo. Neurons within the organoids are functional and exhibit network-like activities. We further demonstrate the utility of the organoid system for modeling the teratogenic effects of Zika virus on the developing brain and identifying new candidate receptors and therapeutic compounds that can mitigate its desructive actions.
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Direct links to NCBI, no account and no request form: the whole study as GSE97919_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 12 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA383338 and SRA study SRP104155. Searching any of these in the dataset finder brings you back here.

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