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Mapping the human DC lineage through the integration of high dimensional techniques

GSE98011 Homo sapiens Expression profiling by high throughput sequencing 93 samples Submitted 2017/05/01 Platform GPL11154
Summary
Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally-specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here we combine two high-dimensional technologies — single-cell mRNA sequencing and Cytometry by Time-of-Flight (CyTOF), to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed sub-populations including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting.
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Direct links to NCBI, no account and no request form: the whole study as GSE98011_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 93 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA383661 and SRA study SRP104402. Searching any of these in the dataset finder brings you back here.

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