The stress granule transcriptome reveals principles of mRNA accumulation in stress granules.
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 3 × U-2 OS Stress Granule RNA vs 3 × U-2 OS Total RNA vs 3 × Yeast SG RNA vs 3 × Yeast Total RNAGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE99304_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA388080 and SRA study SRP108038. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM2640793 U-2 OS Stress Granule RNA replicate 1 U-2 OS Stress Granule R…
- GSM2640794 U-2 OS Stress Granule RNA replicate 2 U-2 OS Stress Granule R…
- GSM2640795 U-2 OS Stress Granule RNA replicate 3 U-2 OS Stress Granule R…
- GSM2640796 U-2 OS Total RNA replicate 1 U-2 OS Total RNA
- GSM2640797 U-2 OS Total RNA replicate 2 U-2 OS Total RNA
- GSM2640798 U-2 OS Total RNA replicate 3 U-2 OS Total RNA
- GSM2640799 Yeast SG RNA replicate 1 Yeast SG RNA
- GSM2640800 Yeast SG RNA replicate 2 Yeast SG RNA
- GSM2640801 Yeast SG RNA replicate 3 Yeast SG RNA
- GSM2640802 Yeast Total RNA replicate 1 Yeast Total RNA
- GSM2640803 Yeast Total RNA replicate 2 Yeast Total RNA
- GSM2640804 Yeast Total RNA replicate 3 Yeast Total RNA
No close match found for this topic, so these are simply the most-viewed datasets of the same kind.
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.