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Western Blot Quantifier
Align your marker, calibrate molecular weight, pick your band, and normalize to a loading control. Every step is logged — all computation runs in your browser.
Initialising Python Runtime
Downloading NumPy + SciPy + tifffile (~15 MB, once per session)…
Have a .scn file? (Typhoon / Cytiva / ChemiDoc)
Just upload it directly — converted automatically in your browser. No server needed.
Resume your previous session?
Uploaded blots and analysis from earlier are saved in this browser.
Normalized Quantification
Figure Labels
| Lane | POI Int. | POI kDa | Ctrl Int. | Ctrl kDa | GOI/Ctrl | Sat% |
|---|
Combine Replicates
Save each biological/technical replicate, then combine them by sample name to get mean ± SEM across blots. All stored in this browser only.
| Condition | Mean | SD | SEM | n |
|---|
Figure Preview
Auto-Generated Methods Text
📑 Cite this tool in your paper
Using this analysis in a publication? Please cite BioTransfer in your Methods section — it keeps the tool free and supports continued development.
Plain text (for Methods section)
Prajapati, B. (2026). BioTransfer Western Blot Quantifier [Browser tool]. Available from https://usebiotransfer.org/tools/western-blot/
BibTeX
@misc{biotransfer_wb_2026,
author = {Prajapati, Bharat},
title = {{BioTransfer Western Blot Quantifier}},
year = {2026},
howpublished = {\url{https://usebiotransfer.org/tools/western-blot/}},
note = {Browser-based tool for reproducible western blot densitometry}
}
Vancouver (numeric style)
Prajapati B. BioTransfer Western Blot Quantifier [Internet]. 2026 [cited ]. Available from: https://usebiotransfer.org/tools/western-blot/
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