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Next-generation sequencing measuring genome-wide RNA decay by s4U Seq in naïve CD4+ T cells from Mettl3 WT and KO mice

GSE100278 Mus musculus Expression profiling by high throughput sequencing 20 samples Submitted 2017/08/17 Platform GPL17021
Summary
Naïve T cells of Mettl3 KO and WT were pulse labeled by s4U for 15 min before and after IL-7 stimulation for 15min/30min/45min/60min/75min/90min. The total input RNAs or s4U nascent RNAs were isolated and prepared for next-generation sequencing. The RNA response to IL-7 and RNA decay rates were then modeled and calculated, to infer the m6A/Mettl3 effects.
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Direct links to NCBI, no account and no request form: the whole study as GSE100278_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 20 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA391351 and SRA study SRP110048. Searching any of these in the dataset finder brings you back here.

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