GEO series

Bovine adult versus prepubertal cumulus cells

GSE10819 Bos taurus Expression microarray 8 samples Submitted 2008/03/18 Platform GPL325

At a glance

Expression microarray · Bos taurus · 8 samples

What it is: Expression microarrayGene activity read on a chip (the older technology that RNA-seq replaced).
Organism: Bos taurus
Samples: 8 samples
Data you can get: A processed expression table (series matrix) and the array files, from NCBI.

Summary (written by the submitters)

The present study was undertaken to discover molecular markers in bovine cumulus cells predictive of oocyte competence and elucidate their functional significance. Differences in RNA transcript abundance in cumulus cells harvested from oocytes of adult versus prepubertal animals (model of poor oocyte quality) were identified by microarray analysis.

Read the full summary ↓

Four genes of interest encoding for the lysosomal cysteine proteinases cathepsin B, S, K and Z and displaying greater transcript abundance in cumulus cells surrounding oocytes harvested from prepubertal animals were chosen for further investigation. Greater mRNA abundance for such genes in cumulus cells of prepubertal oocytes was confirmed by real time RT-PCR. Elevated transcript abundance for cathepsins B, S and Z was also observed in cumulus cells surrounding adult metaphase II oocytes that developed to the blastocyst stage at a low percentage following parthenogenetic activation, versus those that developed at a high percentage. Functional significance of cumulus cell cathepsin expression to oocyte competence was confirmed by treatment of cumulus oocyte complexes during in vitro oocyte maturation with a cell permeable cysteine proteinase (cathepsin) inhibitor. Inhibitor treatment decreased apoptotic nuclei in the cumulus layer and enhanced development of parthenogenetically activated and in vitro fertilized adult oocytes to the blastocyst stage. Stimulatory effects of inhibitor treatment during meiotic maturation on subsequent embryonic development were not observed when oocytes were matured in the absence of cumulus cells. Results support a functional role for cumulus cell cathepsins in compromised oocyte competence and suggest that cumulus cell cathepsin mRNA abundance may be predictive of oocyte quality. Keywords: Bovine, microarray, cDNA, cumulus cells

This dataset

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Download · which file do I need?

Direct links to NCBI, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need.

All supplementary files ↗

3 · The original raw files

Whole study · RAW.tar

Everything the authors deposited as one archive (GSE10819_RAW.tar), including the raw array or instrument files. Can be large; the picker in step 2 takes just the samples you need.

Whole study · RAW.tar ↓

What the files usually look like: For arrays the series matrix is normally the normalised expression table you want; the raw array files (CEL and similar) are in the RAW.tar.

Also filed as BioProject PRJNA107419. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 18417713) ↗

Samples in this study

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+ 8 more — browse all 8 samples with per-sample file links →

Datasets on a similar topic

GSE36605 Transcriptional profiling of cumulus cells differentiation throughout oocyte competence acquisition in the bovine ovarian follicles. 17 samples shares cumulus, bovine
GSE79886 Bovine cumulus cells transcriptome in relation with oocyte chromatin configuration of the germinal vesicle 12 samples shares cumulus, bovine
GSE31261 Transcriptional profiling of cumulus cells entoured the oocyte after in vitro or in vivo maturation in bovine (Bos taurus) 8 samples shares cumulus, bovine
GSE31361 Gene expression profiling of in vitro matured bovine oocytes cultivated with or without cumulus cells. 8 samples shares cumulus, bovine
GSE94338 Effect of low dose docosahexaenoic acid (DHA) on bovine cumulus cells during in vitro maturation 8 samples shares cumulus, bovine
GSE67970 Transcriptome analysis of FSH response in bovine cumulus cells 6 samples shares cumulus, bovine
GSE67969 Transcriptome analysis of bovine cumulus cells during first 6 hrs of in vitro maturation 6 samples shares cumulus, bovine
GSE79760 Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes 18 samples shares cumulus, bovine

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