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The Spatiotemporal Control of Zygotic Genome Activation

GSE122551 Xenopus tropicalis Expression profiling by high throughput sequencing 52 samples Submitted 2019/05/14 Platform GPL21875Platform GPL23182
Summary
One of the earliest and most significant events in embryonic development is zygotic genome activation (ZGA). In several species, bulk transcription begins at the midblastula transition (MBT) when, after a certain number of cleavages, the embryo attains a particular nuclear-to-cytoplasmic (N/C) ratio, maternal repressors become sufficiently diluted, and the cell cycle slows down. Here we resolve the frog ZGA in time and space by profiling RNA polymerase II (RNAPII) engagement and its transcriptional readout. We detect a gradual increase in both the quantity and the length of RNAPII elongation before the MBT, revealing that >1,000 zygotic genes disregard the N/C timer for their activation and that the sizes of newly transcribed genes are not necessarily constrained by cell cycle duration. We also find that Wnt, Nodal, and BMP signaling together generate most of the spatiotemporal dynamics of regional ZGA, directing the formation of orthogonal body axes and proportionate germ layers.
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Direct links to NCBI, no account and no request form: the whole study as GSE122551_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 52 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA505537 and SRA study SRP168643. Searching any of these in the dataset finder brings you back here.

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