← BioTransfer GEO Dataset Finder
GEO series

Embryonic transcription is controlled by maternally defined chromatin state

GSE67974 Xenopus tropicalis Genome binding/occupancy profiling by high throughput sequencing; Methylation profiling by high throughput sequencing; Expression profiling by high throughput sequencing 72 samples Submitted 2015/12/15 Platform GPL20082Platform GPL15472
Summary
During development histone modifying enzymes are required for cell identity and lineage commitment, however little is known about the regulatory origins of the epigenome during embryonic development. Here we generate a comprehensive set of embryonic epigenome reference maps, which we use to determine the extent to which maternal factors shape chromatin state in Xenopus embryos. Using α-amanitin to inhibit zygotic transcription, we find that the majority of H3K4me3 and H3K27me3-enriched regions form a maternally defined epigenetic regulatory space with an underlying logic of hypomethylated islands. This maternal regulatory space extends to a substantial proportion of neurula stage-activated promoters. In contrast, p300-recruitment to distal regulatory regions requires embryonic transcription at most loci. The results show that H3K4me3 and H3K27me3 are part of a regulatory space that exerts an extended maternal control well into post-gastrulation development, and highlight the combinatorial action of maternal and zygotic factors through proximal and distal regulatory sequences.
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE67974_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 72 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA281501 and SRA study SRP057395. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 72 more — browse all 72 samples with per-sample file links →

Similar datasets

Search all ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.